Screening for Functional Microbiota

For culturable microorganisms, the platform is capable of collecting and analysis micro images of colonies in situ on the culture dish. Based on the phenotypic information of these colonies, accurate, objective, and automated "de-replication" of bactria strains can be achieved, greatly improving the efficiency of strain screening.

For uncultured microorganisms, our system enables culture-free, single-cell phenotypic identification and sorting, allowing for the characterization of each microbial cell within its native community.

Utilizing the multi-modal, multi-scale microbial screening platform, we have established a comprehensive solution for microbia screening, encompassing the entire exerimental workflow. This includes sample preparation, single-cell morphological identification, visual-guided and precise single-cell isolation, in situ de-replication colony screening, as well as single cell sequencing and culture. During the sample preparation stage, magnetic nanoparticles can be employed to achieve effective enrichment of low-abundance strains. Subsequently, RAcolony High-Throughput Intelligent Colony Screening System enables higher species recovery with fewer picked colonies.

Meanwhile, for the uncultured microorganisms in the samples, phenotypic identification techniques such as stable isotope probing (SIP) and fluorescence in situ hybridization (FISH) can be used to label targeted microbiota as needed. This is followed by the use of the S3000 Ultra-fast Three-Dimensional Fluorescence Imaging System,  P300 Laser Confocal Raman Spectrometer, PRECI SCS-R300 Raman Single-cell Sorter, or Scatcher Single-Cell Optical Tweezers Manipulation and Sorting System to observe morphology, detect fluorescence singnals, and analysis Raman spectroscopy.  Targeted microbiota can be precisely isolated into lysis buffer, culture medium, or matrix solution for mass spectromery using PRECI SCS Single-cell Sorter or the Scatcher Single-Cell Optical Tweezers Manipulation and Sorting System. This process facilitates single-cell multi-omics research.