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Pathogenic Bacteria Detection

Single-cell Raman Spectroscopy Integrated with Deep Learning for the Rapid Classification and Identification of Pathogenic Microorganisms.

 

Conventional pathogenic bacteria detection primarily relies on culture-based methods or molecular biology techniques, which are time-consuming and labor-intensive. Raman spectroscopy, as a high-resolution and highly sensitive detection method, enables "culture-free" pathogenic bacteria detection at the single-cell level, significantly improving detection speed. 


The P300 Confocal Raman Spectrometer enables the detection of single pathogenic bacteria. In addition, by leveraging the diverse machine learning and deep learning algorithms offered by the HOOKE IntP intelligent data analysis software, the types of pathogenic bacterial species can be rapidly and accurately predicted. Moreover, by integrating with the PRECI SCS Single-cell Sorter (or using the PRECI SCS-R300 Raman Single-cell Sorter), targeted individual pathogenic bacteria can be isolated for multi-omics analysis, facilitating further investigation its role in the physiological and pathological processes of the host.

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    Culture-free
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    Label-free
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    High Sensitivity
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    Low Cost

Classification of Pathogenic Bacteria Based on Raman Spectroscopy and Deep Learning

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Image Source:Bo Liu et al., Journal of Biophotonics, 2022

Single-cell Raman spectra, often known as the "molecular fingerprint" of microorganisms, holds significant value for species identification. 


The P300 Confocal Raman Spectrometer can efficiently collect Raman spectral data from various types of pathogenic bacteria at the single-cell level. Deep learning algorithms, such as neural networks, are integrated to develop a classification and identification model. This model is then applied to predict the types of pathogenic bacteria in samples, significantly shortening the analysis period.

Classification of Gram-negative and Gram-positive Bacteria Based on Raman Spectroscopy integrated with Machine Learning

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Image Source: Huijie Hu et al., Analytical Methods, 2022

The P300 Confocal Raman Spectroscopy is used to collect Raman spectra from various  common Gram-negative and Gram-positive bacteria. Following, the collected Raman specral data are trained using machine learning algorithms (such as SVM) within the HOOKE IntP intelligent analysis software to establish a Gram staining (GS) classification and identification model. This enable accurate and rapid prediction of bacterial GS types.

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