DonutFlick
Versatile Single-Cell Sorter
DonutFlick integrates laser-assisted gentle sorting with high-resolution microscopic imaging,enabling real-time visualization of cellular morphology and fluorescence signals during the sorting process. Whether working with valuable primary-cell suspensions or spatially resolved tissue sections, DonutFlick delivers precise single-cell isolation with recovery rate exceeding 95% while maintaining high cell viability. By preserving critical cellular information throughout the workflow, the system enables seamless integration with downstream sequencing and cell culture applications, bridging phenotype-based selection with genotype-driven analysis.
Brochures
Advantages
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Compatible with both cell suspensions and tissue sections: One System, Multiple UsesWidely applicable to diverse sample types, including cell suspensions, formalin-fixed paraffin-embedded (FFPE) tissue sections, and cryosections, fully satisfying multidimensional single-cell research needs.
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High Single-Cell Recovery Rate & Preserved Cell ViabilityBased on the principle of laser-induced forward transfer (LIFT), the single-cell recovery rate remains stable above 95%, and the cell survival/culture success rate exceeds 90%, providing high-quality samples for downstream investigation.
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New Strategies for Rare and Low-Abundance Cell SortingIdeal for low cell-density samples (as low as 10³ cells/mL) and rare cell populations (representing less than 0.1% of total samples), breaking through the limitations of conventional single-cell research.
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Visualized Precision Sorting with Full-Process Data TraceabilityEquipped with a research-grade microscopic imaging system and a 4-channel fluorescence module, the entire sorting process is visually recorded. The image, coordinates, and fluorescence intensity of every single cell can be fully traced.
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Highly Automated and User-Friendly OperationFeatures an integrated workflow covering wide-field scanning and image stitching, automatic focusing, AI-powered single-cell image recognition, one-click automated detection and isolation, and automated cell collection, significantly reducing experimental burdens and enhancing research efficiency.
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Seamless Integration with Downstream Multi-Omics StudiesIsolated cells can be directly utilized for single-clone culture, single-cell genomic amplification and sequencing, and single-cell proteomic analysis, effectively bridging phenotype-based selection with genotype-driven analysis.
Working Principle
Utilizing Laser-Induced Forward Transfer (LIFT) technology combined with a beam shaping system to form a 'donut-shaped' laser beam, enabling precise non-contact separation of single cells and subcellular structures.
Applications
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Medical ResearchPhenotypic detection combined with single-cell analysis enhances detection speed and accuracy, driving advancements in medical research.
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BiopharmaceuticalsFrom early-stage screening to formulation development, driving innovation in the biopharmaceutical field.
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Medical Research
Phenotypic detection combined with single-cell analysis enhances detection speed and accuracy, driving advancements in medical research.
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Biopharmaceuticals
From early-stage screening to formulation development, driving innovation in the biopharmaceutical field.
Product Parameters
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Functional Indicator
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Sortable cell types
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Sortable cell size
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Single-cell recovery rate
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Cell culture success rate
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Minimum sample concentration
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Cell collection
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Microscopic imaging
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Intelligent recognition functions
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Accessories
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Downstream applications
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Specification
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Animal cells, tissue sections, plant cells, microorganisms, etc.
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1-30 μm
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≥95%
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≥90%
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1×10³ cells/mL
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96-well plate, 8-strip microwell receiver
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Research-grade brightfield imaging; 4-channel fluorescence imaging: DAPI, GFP, Cy3, Cy5; more wavelengths can be customized.
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Morphological parameter analysis, fluorescence-intensity analysis and recognition, automatic numbering and localization.
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Clean bench, microfluidic control device.
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Cell culture, genomics, transcriptomics, proteomics, qPCR, etc.
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