On September 26, 2022, the team of Academician Yongguan Zhu and Researcher Li Cui from Institute of Urban Environment, Chinese Academy of Sciences, applied PRECI SCS, the core product of HOOKE INSTRUMENTS, The paper is titled"Active antibiotic resistome in soils unraveled by single-cell isotope probing and targeted metagenomics" in PNAS .The first author is Dr. Hongzhe Li. The paper traces active antibiotic resistant bacteria in soil in situ and quantifies their phenotypic resistance level by developing single-cell Raman stable isotope labeling and targeted metagenomic combination technology. Combined with single cell targeted sorting and sequencing, the resistance group and mobile group of soil highly active drug-resistant bacteria were revealed.

Abstract

Antimicrobial resistance (AMR) in soils represents a serious risk to human health through the food chain and human-nature contact. However, the active antibiotic-resistant bacteria (ARB) residing in soils that primarily drive AMR dissemination are poorly explored. Here, single-cell Raman-D2O coupled with targeted metagenomics is developed as a culture-independent approach to phenotypically and genotypically profiling active ARB against clinical antibiotics in a wide range of soils. This method quantifies the prevalence (contamination degree) and activity (spread potential) of soil ARB and reveals a clear elevation with increasing anthropogenic activities such as farming and the creation of pollution, thereby constituting a factor that is critical for the assessment of AMR risks. Further targeted sorting and metagenomic sequencing of the most active soil ARB uncover several uncultured genera and a pathogenic strain. Furthermore, the underlying resistance genes, virulence factor genes, and associated mobile genetic elements (including plasmids, insertion sequences, and prophages) are fully deciphered at the single-cell level. This study advances our understanding of the soil active AMR repertoire by linking the resistant phenome to the genome. It will aid in the risk assessment of environmental AMR and guide the combat under the One Health framework.

The paper links：

https://www.pnas.org/doi/10.1073/pnas.2201473119